Tryptophan synthase and production of L-tryptophan in regulatory mutants.
نویسندگان
چکیده
منابع مشابه
Production of a novel tryptophan analog, beta-1-indazole-L-alanine with tryptophan synthase of Escherichia coli.
The tryptophan synthase alpha 2 beta 2 complex from Escherichia coli has been found to catalyze the beta-replacement reaction of L-serine with indazole, an indole analog which has a nitrogen atom at the 2-position (pyrazole ring). The reaction product was isolated and identified as beta-indazolealanine by mass spectrometric, elemental and NMR analyses. Careful assignment of 1H- and 13C-signals ...
متن کاملModification of tryptophan transport system and its impact on production of L-tryptophan in Escherichia coli.
The production of L-tryptophan through chemical synthesis, direct fermentation, bioconversion and enzymatic conversion has been reported. However, the role of transport system for aromatic amino acids in L-tryptophan producing strains has not been fully explored. In this study, the fact was revealed that L-tryptophan production and cell growth were affected by the modification of transport syst...
متن کاملRegulatory mutants of the tryptophan operon of Salmonella typhimurium.
HE tryptophan ( t rp) operon of Salmonella typhimurium consists of five Tstructural genes regulated as a unit by tryptophan (BLUME and BALBINDER 1966; BAUERLE and MARGOLIN 1966a, 196613; BLUME, WEBER and BALBINDER 1968). The biosynthetic reactions involved and the order of the genes (see Figure 1 ), as well as the regulation of the trp operon have been shown to be identical in Escherichia coli ...
متن کاملl-Tryptophan Production by Achromobacter liquidum.
Conditions for the production of tryptophanase from Achromobacter liquidum and for the conversion of l-serine and indole to l-tryptophan were studied. The enzyme could be produced in amounts as great as 0.750 U/ml (degradation) and 0.294 U/ml (synthesis) by shaking cultures at 30 degrees C in a medium containing dextrin, yeast extract, l-tryptophan, and l-glutamic acid. l-Tryptophan was produce...
متن کاملL-Tryptophan Production by Auxotrophic and Analogue Resistant Mutants of Aureobacterium flavescens
A number of tyrosine plus phenylalanine double auxotrophic mutants were isolated by N-methyl-N-nitro-N-nitrosoguanidine (MNNG) treatment of a locally isolated strain of Aureobacterium flavescens of which 11A(39) and 11A(17) were selected on the basis of their tryptophan production in a mineral salt medium over other isolated mutant strains. The mutational block in the aromatic amino acid biosyn...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1982
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.46.2711